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Part:BBa_J70026

Designed by: Tom Knight   Group: Knight Lab   (2008-10-20)
Revision as of 17:02, 6 May 2009 by Tk (Talk | contribs) (→‎Usage and Biology)

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cat cassette (chloramphenicol resistance)

cat gene and promoter from pC194 plasmid, Genbank V01277.

Usage and Biology

MIC for E. coli DH10B cells is 4 ug/ml chloramphenicol. This is near background; the cassette appears not to function in E. coli. Horinouchi (1982) says this cassette is inducible. Growth at 0.5 ug/ml for 30 minutes, followed by resistance at 20 ug/ml in E. coli. See part J70304 for a version with the 5' UTR eliminated in an effort to eliminate this behavior.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/mflorum
Parameters
None