Protein_Domain

Part:BBa_K4033008

Designed by: Jiaying Hou   Group: iGEM21_BHSF   (2021-10-01)
Revision as of 05:47, 19 October 2021 by EchoH (Talk | contribs)


antE

AntE is extracted from Photorhabdus luminescens. It is a component of the type II PKS system that can produce octaketides in E.coli.

Biology and Usage

The entire mPKS complement from P. luminescens, comprising the KS AntD, CLF AntE, and ACP AntF, were successfully expressed as soluble recombinant proteins in E. coli. Whilst AntD is a soluble recombinant proteins in E. coli, the role of AntE as a functional heterodimer partner for AntD was unknown. Sequence features of AntE defy conventions of characterised CLFs: alignment of CLF amino acid sequences in our dataset showed AntE to fringe the clade of canonical CLFs and to also lack hallmark and gatekeeper residues that play important roles in polyketide biosynthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None