Part:BBa_K3803016

This is a efficient xylose utilization composite part.

This is an efficient xylose utilization BBa_K3803016 we built based on the parts BBa_K2314913 & BBa_K2314324 (Although they were uploaded as basic parts separately, they worked together as a composite part) from OUC_China in 2017 iGEM.

Xylose reductase (XR) will first transform xylose to xylitol, and xylitol dehydrogenase (XDH) can further convert xylitol to xylulose. Then, xylulose will be converted to xylulose 5-phosphate (X5P) by the native xylulose kinase (XK). Apart from the original XR and XDH genes, considering the low copy number of native XK gene in S. cerevisiae, we introduced an extra XK gene to improve the xylose utilization ability.

For cell growth, OUC_China achieved a final OD₆₀₀ around 2.35 in YNB-based xylose media. After our improvement on their parts, we could get a final OD₆₀₀ around 20.

Fig.1 The OD₆₀₀ Value with Different Xylose Utilization Genes

For our own product yield, our production also improved after substituting our three-gene cassette for OUC_China's two-gene one. The improved production was very obvious after the parts improvement based on our HPLC results.

Fig.1 The HPLC Results with Different Xylose Utilization Genes

For more information about our parts and improvement, you can click our page.

Sequence and Features BBa_K3803016 SequenceAndFeatures