Composite

Part:BBa_J70303

Designed by: Tom Knight   Group: Knight Lab   (2009-04-20)
Revision as of 22:55, 5 May 2009 by Tk (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Test Cm resistance plasmid for Mesoplasma florum

Assembly of pSCI2 plasmid origin and cat cassette into E. coli plasmid pSB1T3.

Usage and Biology

MIC in E. coli DH10B cells is 1 ug/ml of chloramphenicol. This is near background; the cassette appears not to function in E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 372


[edit]
Categories
//chassis/prokaryote/mflorum
Parameters
None