Coding

Part:BBa_K3745002:Design

Designed by: Jiaqi Tang   Group: iGEM21_BNDS_China   (2021-09-29)
Revision as of 04:16, 19 October 2021 by Jianfei Song (Talk | contribs) (→‎Design Notes)

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rhlB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
    Illegal AgeI site found at 859
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

To facilitate genetic engineering, the endogenous enzymes rhlB is transformed from P.Aeruginosa into E. coli because E. coli is a model organism and is well-researched.

References

Du, J., Zhang, A., Hao, J., & Wang, J. (2017). Biosynthesis of di-rhamnolipids and variations of congeners composition in genetically-engineered Escherichia coli. Biotechnology Letters, 39(7), 1041–1048. https://doi.org/10.1007/s10529-017-2333-2

Gong ZJ, Peng YF, Zhang YT, et al. Construction and optimization of Escherichia coli for producing rhamnolipid biosurfactant. Chin J Biotech, 2015, 31(7): 1050–1062.