Part:BBa_I12219

Wild-type TetR(B) promoter (negative)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization by 2021iGEM_Shanghai_Metro

BBa_K4028005

Name: tet promoter

Base Pairs: 56bp

Origin: Escherichia coli, synthetic

Properties: Inducible promoter regulated by tetracycline

Usage and Biology

We set different tetracycline concentrations to test the protein expression induced by TetR(B) promoter. In our design, the immune protein (ike4) is regulated by TetR(B) promoter. The expression of the immune protein (ike4) directly affects and is positively correlated with strain growth. Therefore, the strain growth can indicate the regulation of the TetR(B) promoter.

The results of the test are below.

Table 1. OD600 value of the strain D: Pus232-ike4-tke4 after 6 hours

Figure 1. Biomass Concentration curve of Pus323-tke4-ike4 Strain with tetracycline

The results show that concentrations at the range of 300-500µg/L are more effective. The results also proved that adding tetracycline to strain will boost the growth of strain. It determines that the optimal concentration of tetracycline is 300µg/L to 500µg/L.