Part:BBa_K4046100:Design

DhdO Binding Site #1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This gene was synthesized commercially from the published sequence (IDT). For an initial proof-of-concept, we introduced the binding sites into a commercially available pcDNA5 plasmid (Thermo Fischer, V103320) with mCherry fluorescence protein. HEK cells were transfected with these plasmids and imaged for fluorescent activity.

Figure 1: Fluorescent microscopy results of HEK293T cells transfected with our binding site plasmids.

Source

This is an identified binding site for the DhdR gene, and is generated from sequence analysis.

References

A D-2-hydroxyglutarate biosensor based on specific transcriptional regulator DhdR Dan Xiao, Wen Zhang, Xiaoting Guo, Yidong Liu, Chunxia Hu, Shiting Guo, Zhaoqi Kang, Xianzhi Xu, Cuiqing Ma, Chao Gao, Ping Xu bioRxiv 2021.02.18.430539; doi: https://doi.org/10.1101/2021.02.18.430539