Composite

Part:BBa_K3738009

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)
Revision as of 02:47, 19 October 2021 by Emily Hagens (Talk | contribs)


MlrA (Microcystinase) Composite Part

MlrA, or microcystinase, cleaves the peptide backbone of MC-LR cyanotoxins produced by microcystis aeruginosa cyanobacteria. This crucial step results in the peptide losing the cyclic structure, and therefore the majority of its toxicity. Further degradation continues in an enzymatic pathway by interactions with other peptidolytic enzymes from the microcystinase gene cluster. This composite part includes the IPTG-inducible T7 promoter (BBa_J64997), RBS BBa_B0034, microcystinase coding region BBa_K3738019, and double terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 556
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None