Coding

Part:BBa_K3738019

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-18)
Revision as of 02:38, 19 October 2021 by Registry (Talk | contribs)

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MlrA for E.coli

MlrA, or microcystinase, cleaves the peptide backbone of MC-LR cyanotoxins produced by microcystis aeruginosa cyanobacteria. This crucial step results in the peptide losing the cyclic structure, and therefore the majority of its toxicity. Further degradation continues in an enzymatic pathway by interactions with other peptidolytic enzymes from the microcystinase gene cluster. This part originally comes from the Aalto-Helsinki 2016 iGEM team (Part BBa_K1907001), but was originally codon-optimized for yeast. We have modified this part by codon-optimizing it for use in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 868
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 868
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 868
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 868
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 868
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None