Composite
ara_gp5.7

Part:BBa_K3790232:Design

Designed by: Chongwen Cao   Group: iGEM21_Fudan   (2021-10-17)
Revision as of 00:33, 19 October 2021 by Chongwen (Talk | contribs) (→‎Design Notes)


T7 gene product 5.7 driven by pBAD/araC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The following primers were used for constructing this part. 

doubleter_f	5-CGATATAAGTTGTAATTCTCaaataataaaaaagccggattaataatctggc
doubleter_r	5-GAAGGCTCTCAAGGGCATtcacactggctcaccttcg
gp5.7_f		5-gaATGCCCTTGAGAGCCTTCTTAGACACATCCTCCCATTCGTTTC
gp5.7_r		5-gagttaaggaggtaaaaaaaATGTCTGACTACCTGAAAGTGCTG
pBAD_f		5-tttttttacctccttaactctagtagctagcc
pBAD_r		5-TGGTGCTACGCCTGAATAAGttatgacaacttgacggctacatcattc
pLysS_f		5-tagccgtcaagttgtcataaCTTATTCAGGCGTAGCACCAG
pLysS_r		5-GAGAATTACAACTTATATCGTATGGGGC
gp5.7_coding1  	GTCCAACTATGTACGGAACAATGCGAGCCTCGTAGCGGAGGCCGCTTCCCGTGGTCAC 	
gp5.7_coding2  	CTGCAAGCAATCAAAAGTTGCCCTAAGACTTTCCAGTCCAACTATGTACGGAACAATGC	
gp5.7_coding3  	ATGTCTGACTACCTGAAAGTGCTGCAAGCAATCAAAAGTTGCC                	
gp5.7_template1	CCCAAGCGCCACCGTTACGTCCACTAGTAGTCAGGCACGAGATGTGACCACGGGAAGCG	
gp5.7_template2	CATTCGTTTCAGAAAGCGAGTACCGGAAGCAGTGATTTCCCAAGCGCCACCG       	
gp5.7_template3	TTAGACACATCCTCCCATTCGTTTCAGAAAGCGAGTACC       
f_gp5.7_rep2_1722	ggtttactttcgtccgcgcgcc          	
f_gp5.7_rep2_1869	GGCACTGATGAGGGTGTCAGTGAAGTGC    	
r_gp5.7_rep2_1722	CTGACACCCTCATCAGTGCCAACATAGTAAGC	
r_gp5.7_rep2_1869	cgcgcggacgaaagtaaacccac

Source

References