Part:BBa_K3889132

Bacillus subtilis BBa_B0010-nagP fused terminator check cassette

Cassette for checking BBa_B0010 and Bacillus subtilis native terminator nagP using dual reporter system.
Fig 1. Spacer replaced from BBa_K3889130 by BBa_B0010

Formulae for terminator efficiency [1]:

where

mCherry₀ → mCherry produced by device without terminator

sfGFP₀ → sfGFP produced by device without terminator

Using the device (BBa_K3889130) without any changes, TE_Device can be calculated, which gives the expression of mCherry in the absence of a terminator.

where

mCherry → mCherry produced by the device with terminator

sfGFP → sfGFP produced by the device with terminator

and TE → is the terminator efficiency of the BBa_K3889070

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 121
  

References

1. Gale, G. A. R., Wang, B., & McCormick, A. J. (2021). Evaluation and Comparison of the Efficiency of Transcription Terminators in Different Cyanobacterial Species. Frontiers in Microbiology, 11. https://doi.org/10.3389/fmicb.2020.624011