Part:BBa_K3771079

T7-JJU

Description

P_T7-jju is a composite part consisting of the T7 promoter and the cs sequences. This part was used in in vivo testing of taurine production. The sequence for jju and T7 promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Biology

T7 promoter constitutively facilitates the expression of JJU enzyme.

圖片描述

Fig 1. Confirmation of jju fragment by PCR. M: Marker; Lane 1: jju (1180bp)

Fig 2. Confirmation of pET28a fragment by digestion. M: Marker; Lane 1: pET28a (~5280 bp)

Fig. 3 Transformation/JJU in DH5α

Fig. 4 Confirmation of pET28a-T7-JJU by digestion. M: Marker; Lane 1: Colony of pET28a-T7-JJU (~6466 bp)

Fig. 5 Transform into BL21(DE3)

Fig. 6 Confirmation of protein expression of JJU. M: Marker; Lane1: whole cell of JJU in BL21(DE3); Lane2: soluble protein of JJUBL21(DE3) (~43 kDa)

Fig. 7 Transform into BD7G

Fig. 8 Confirmation of protein expression of JJU. M: Marker; Lane1: whole cell of JJU in BD7G; Lane2: soluble protein of JJU in BD7G(~43 kDa)

Usage

Characterization

References
Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]