Coding

Part:BBa_K3900021

Designed by: Lucas Krause   Group: iGEM21_Bielefeld-CeBiTec   (2021-09-22)
Revision as of 19:38, 18 October 2021 by Lucas Krause (Talk | contribs)


emission592 nm
excitation460 nm

mCRISPRed - improvement of mRuby3

This part is an improved version of the red fluorescent protein mRuby3 (BBa_M50009), that displays an enhanced stability at low pH conditions. The original part can be found at: https://parts.igem.org/Part:BBa_M50009

Measurement
The improved pH stability was determined by measuring the relative fluorescence of mRuby3 and mCRISPRed at different pH conditions. The fluorescence proteins were expressed in Escherichia coli BL21 DE3 and purified via IMAC beforehand.

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Categories
Parameters
emission592 nm
excitation460 nm