Part:BBa_K3889050
Bacillus subtilis TAT signal peptide PhoD
Alkaline Phosphatase D is one of the many extracellularly secreted proteins found in Bacillus subtilis. It is preceded by a signal peptide sequence of 56 AA which targets the folded protein to the extracellular space via Twin Arginine Translocase Ay-Cy and Twin Arginine Translocase channels in B.subtilis. It has also been thought to use Sec secretory pathway in Bacillus subtilis for unfolded protein secretion. Although the efficiency of secretion is ultimately dependant on multiple factors including conformation of the protein, cellular environment, etc. this part might be used for secretion of different recombinant proteins in Bacillus subtilis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1. Kolkman, M. A., van der Ploeg, R., Bertels, M., van Dijk, M., van der Laan, J., van Dijl, J. M., & Ferrari, E. (2008). The twin-arginine signal peptide of Bacillus subtilis YwbN can direct either Tat- or Sec-dependent secretion of different cargo proteins: secretion of active subtilisin via the B. subtilis Tat pathway. Applied and environmental microbiology, 74(24), 7507–7513. https://doi.org/10.1128/AEM.01401-08
2. Peng, C., Shi, C., Cao, X., Li, Y., Liu, F., & Lu, F. (2019). Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria: Signal Peptide and Beyond. Frontiers in bioengineering and biotechnology, 7, 139. https://doi.org/10.3389/fbioe.2019.00139
3. Frain, K. M., van Dijl, J. M., & Robinson, C. (2019). The Twin-Arginine Pathway for Protein Secretion. EcoSal Plus, 8(2), 10.1128/ecosalplus.ESP-0040-2018. https://doi.org/10.1128/ecosalplus.ESP-0040-2018
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