Regulatory
Part:BBa_K4020004
Designed by: Karina Kurbanova Group: iGEM21_Estonia_TUIT (2021-10-18)
Regulatory
Usage and Biology
LexA is a synthetic promoter that contains four LexA boxes. LexA boxes are parts derived from the bacterial LexA promoter and contain two SOS motifs. Additionally, this synthetic promoter has a core of the modified CYC1 promoter from Saccharomyces cerevisiae. Synthetic LexA promoter can be recognised by transcription factor LexA-DNA binding domain to control the expression of the successive gene (Ottoz et al., 2014). It was acquired from the DualMembrane Kit 3 (Thaminy et al., 2003).
References
- Ottoz, D. S. M., Rudolf, F., & Stelling, J. (2014). Inducible, tightly regulated and growth condition-independent transcription factor in Saccharomyces cerevisiae. Nucleic Acids Research, 42(17), e130–e130. https://doi.org/10.1093/NAR/GKU616
- Thaminy, S., Auerbach, D., Arnoldo, A., & Stagljar, I. (2003). Identification of Novel ErbB3-Interacting Factors Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System. Genome Research, 13(7), 1744. https://doi.org/10.1101/GR.1276503
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