Part:BBa_K3952000

APOB-100 (Wild Type)

Located within chromosome 11 of the Bos taurus genome, the APOB gene codes for apolipoprotein B, a protein involved in the transport of cholesterol in the bloodstream. Studies have reported an inheritable allele within the APOB gene that causes cholesterol deficiency (CD) in cattle. This part contains the wild-type APOB gene with nucleotides CT at positions 221, 222. Part: BBa_K3952001 contains a transposable insertion between nucleotides CT at positions 221, 222. The insertion leads to cholesterol deficiency within the animal.

BBa_K3952001

The APOB gene (Gene ID 494004) was located within the genomic DNA sequence of the Bos taurus, using the NCBI reference assembly.

Usage and Biology

The purpose of this part is to serve as a positive control for a Flappase genetic testing system. The system requires the amplification of DNA around the polymorphism, therefore the sequence provide is only a fragment of the Bos taurus genome. The sequence was cut in order to only showcase where the allele would be present (exon 5) but allow ease of cloning.

This part does not contain the APOB gene allele.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]