Regulatory
Part:BBa_K4092006:Design
Designed by: Emiliano González Castañón, Juan Antonio Alfaro Almaguer & Emilio Fabian Ortiz Group: iGEM21_Tec-Monterrey (2021-10-18)
Toehold for Fusarium oxysporum 1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed with Toehold Switch Creator. We added two bases arbitrarily to the linker sequence in order for our reporter genes to be in frame with the start codon in the hairpin after assembly.
Source
RNA complementary to the 28S-18S ribosomal RNA intergenic spacer sequence from fusarium oxysporum