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Part:BBa_K3989008
Engineered quorum sensing esaR promoter: PesaR-C
A promoter that can interact with EsaR protein and inhibit downstream gene transcription.
Basic information
This part represents a promoter(Pesa) variant with two esa box, which can be bound to EsaR protein(see figure 2). esa box is a 18bps period located at a specific position of the promoter. After binding to the box, the EsaR will repress the recruitment of the RNA polymerase and inhibit the downstream transcription. However, when there are AHL molecules(such as 3OC6HSL) existing in the environment, they will interact with the EsaR and release it from the promoter, thus the transcription can be started.(Figure 1) The transcription level is controlled by the concentration of the AHL molecules.
<. >Figure 3.Gene expression controlled by AHL molecules, EsaR regulator and PesaR promoter. On the left side, the test is based on the PesaR promoter and on the right side, the test is based on the PesaRC promoter. If one use 10nM Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 281
Illegal XhoI site found at 1 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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