Coding

Part:BBa_K3927017

Designed by: Chew Chin Wei   Group: iGEM21_NUS_Singapore   (2021-10-13)
Revision as of 14:07, 18 October 2021 by CWChew (Talk | contribs) (→‎Characterization)

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Human Beta Defensin 2 (S. cerevisiae)

Coding sequence for the human immune protein, human beta defensin 2. Codon optimized for S. cerevisiae).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

This part contains the CDS for Human Beta Defensin 2 (HBD2) with a 6xHis-tag attached to the C-terminus of the peptide sequence.

Usage

This part contains only the CDS and 6xHis-tag. Stop codon is required at the end of the sequence as it is not included.

Promoter and terminator is required for successful expression, additional secretion signal peptide may be required to successfully express and purify HBD2 from culture.

Design

This part was derived from the gene encoding for Human Beta Defensin 2 (DEFB4). The part sequence has been codon optimized for expression in S. cerevisiae and P. pastoris.

Characterization

Part characterization was performed using the composite part BBa_K3927018 (HB2 with mating factor alpha secretion tag). Please refer to that part page for more details.

Please also refer to the NUS Singapore 2021 results page for more information on this part and the detailed characterization data using this link: https://2021.igem.org/Team:NUS_Singapore/Results


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Categories
Parameters
None