Part:BBa_K3763001
Over-expression of FadR
In the absence of fatty acids, an intrinsic transcription factor FadR acting as the repressor in the fatty acid metabolism system in E. coli. will attach to the promoter, and block the downstream gene expression.
To better reduce expression leakage, we overexpressed FadR, which has been shown effective to increase bacterial sensitivity to fatty acids. Characterization We cloned genomic DNA FadR from E.coli MG1655 and digested it with HindIII and XbaI. Plasmid (pBAD33)was extracted using an extraction kit, and digested with HindIII and XbaI as well. Right bands of pBAD33 vector (around 5000 bp) and FadR gene sequence (1038bp) were observed.
The gene sequencing results indicated that we successfully get FadR.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
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