Composite

Part:BBa_K3852008

Designed by: YiYang Sun   Group: iGEM21_BIT-China   (2021-10-01)
Revision as of 13:48, 18 October 2021 by Caoyuan (Talk | contribs)


Pfba1+T1R1+eGFP+CYC1t

The main function of this part is to express T1R1 and detect its localization on the cell membrane of SACCHARomyces cerevisiae.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3122
    Illegal XbaI site found at 646
    Illegal XbaI site found at 1485
    Illegal XbaI site found at 1575
    Illegal XbaI site found at 1893
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3122
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3122
    Illegal BglII site found at 849
    Illegal BglII site found at 2451
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3122
    Illegal XbaI site found at 646
    Illegal XbaI site found at 1485
    Illegal XbaI site found at 1575
    Illegal XbaI site found at 1893
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3122
    Illegal XbaI site found at 646
    Illegal XbaI site found at 1485
    Illegal XbaI site found at 1575
    Illegal XbaI site found at 1893
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2071


[edit]
Categories
Parameters
None