Composite

Part:BBa_K4088888

Designed by: Ruslan Vasiliev, Yaroslav Ugolkov, Alice Potapova, Pavel Ivanov-Rostovtsev   Group: iGEM21_Moscow_City   (2021-10-18)
Revision as of 13:04, 18 October 2021 by Ugol24d (Talk | contribs)


dCas13a-Clact

С-terminal fragment of β-lactamase fused with dCas13a.

β-Lactamase (AmpR) -
C-terminal fragment of β-lactamase -

dCAS13 is a catalytically dead Cas13a enzyme(formerly C2c2). The Cas13 family of enzymes includes at least 4 families, for example Cas13a, which is used in this biobrick. dCas13a interacts with gRNA about 65 nucleotides in length. dCas13a protein complexes with gRNA detect complementary RNA in the sample. dCas13a is very specific variant of RNA detection, it can been customized to recognize single nucleotides on the target RNA.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1580
    Illegal BglII site found at 1790
    Illegal BglII site found at 2057
    Illegal BglII site found at 2822
    Illegal XhoI site found at 3751
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None