Part:BBa_K4088888

dCas13a-Clact

С-terminal fragment of β-lactamase fused with dCas13.
β-Lactamase (AmpR) -
C-terminal fragment of β-lactamase -

dCAS13 is a catalytically dead Cas13a enzyme(formerly C2c2). The Cas13 family of enzymes includes at least 4 families, for example Cas13a, which is used in this biobrick. dCas13a interacts with gRNA about 65 nucleotides in length. dCas13a protein complexes with gRNA detect complementary RNA in the sample. dCas13a is very specific variant of RNA detection, it can been customized to recognize single nucleotides on the target RNA.

This biobrick, together with BBa_K4088889, should effectively detect even small amounts of RNA in the sample.

<a href="https://parts.igem.org/Part:BBa_K4088889">BBa_K4088889</a>

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1580
  Illegal BglII site found at 1790
  Illegal BglII site found at 2057
  Illegal BglII site found at 2822
  Illegal XhoI site found at 3751
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]