Composite

Part:BBa_K4088888

Designed by: Ruslan Vasiliev, Yaroslav Ugolkov, Alice Potapova, Pavel Ivanov-Rostovtsev   Group: iGEM21_Moscow_City   (2021-10-18)
Revision as of 12:44, 18 October 2021 by Ugol24d (Talk | contribs)


dCas13a-Clact

С-terminal fragment of β-lactamase fused with dCas13.
β-Lactamase (AmpR) -
C-terminal fragment of β-lactamase -

dCAS13 is a catalytically dead Cas13 enzyme. The diverse Cas13 family contains at least four known subtypes, including Cas13a (formerly C2c2), which we used. Cas13 uses ~ 64 nucleotide guide RNA to encode target specificity. Cas13 protein complexes with gRNA detect complementary RNA in the sample. Cas13 based detection is specific and can be customized to recognize single nucleotides on the target RNA.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1580
    Illegal BglII site found at 1790
    Illegal BglII site found at 2057
    Illegal BglII site found at 2822
    Illegal XhoI site found at 3751
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None