Coding

Part:BBa_K3989008

Designed by: Tianyu Xu   Group: iGEM21_UZurich   (2021-09-30)
Revision as of 12:40, 18 October 2021 by LHospital (Talk | contribs)


Engineered quorum sensing esaR promoter: PesaR-C

A promoter that can interact with EsaR protein and initiate downstream gene transcription.

Basic information

This part represents a promoter variant with two esa box(position -10 & between -10, -35), which can be bound to EsaR protein. After binding to the box, the EsaR will recruit the RNA polymerase and regulate the downstream transcription. The original promoter: PesaR has only one esa box located at position -10 and shows a higher transcription efficiency. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 281
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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