Coding

Part:BBa_K3740009

Designed by: Guiyi Huang   Group: iGEM21_SZPT-CHINA   (2021-08-26)
Revision as of 11:58, 18 October 2021 by HUANG (Talk | contribs) (→‎2021 SZPT-China)


Holin S

Description

Lysis of the engineered bacteria

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


2021 SZPT-China

Biology

The S gene of lambda bacteriophage has the characteristics of a double initiation motif, which can encode two proteins with opposite functions, S107 and S105. Among them, S105 plays the function of holin. It accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space.

References

[1] Yibo S, Jianhe S, Shi Y, et al. Current advance in the topological structure and function of holin encoded by bacteriophage lambda[J]. Acta Microbiologica Sinica, 2012, 52(2):141-145

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Categories
Parameters
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