Coding

Part:BBa_K3784002:Design

Designed by: LUXUAN LIU   Group: iGEM21_BNUZ-China   (2021-09-19)
Revision as of 08:00, 18 October 2021 by MANUL (Talk | contribs) (→‎Design Notes)


fldC (Encoding 2-hydroxyacyl-CoA dehydratase family protein)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 799
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 799
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 799
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use gibson recombination to ligate it to the plasmid.

Source

fld gene cluster of Clostridium sporogenes

References