RNA

Part:BBa_K3843006:Design

Designed by: Anthony Dang   Group: iGEM21_Waterloo   (2021-10-01)
Revision as of 07:33, 18 October 2021 by Anthneedee (Talk | contribs) (→‎Source)


LbuCas13a guide RNA - DRD4-targeting (missense deletion in VNTR motif 3)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To ensure binding specificity for the Cas13a variant from Leptotrichia buccalis, the stem region was adapted from Fozouni et al. (2020), which used the same stem region successfully when designing guide RNAs for the detection of SAR-CoV-2 RNA.

The recognition sequence was adapted from (CITE), where a 34-bp region containing the ADHD-associated mutation of interest was selected.

The sequence was then converted to DNA (converting all U bases to T) in order to be submitted to the Parts Registry.

Source

The stem (hairpin stem+loop region) of the guide RNA was obtained from Fozouni et al. (2020) (GIVE REFERENCE).

The DRD4-mutant-targeting recognition sequence of the guide RNA was obtained from ____________

References