Coding

Part:BBa_K1159105:Experience

Designed by: Dong-Jiunn Jeffery TRUONG   Group: iGEM13_TU-Munich   (2013-09-14)
Revision as of 06:34, 18 October 2021 by CWChew (Talk | contribs) (→‎User Reviews)


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Applications of BBa_K1159105

User Reviews

The Sao Carlos-Brazil Team has developed a codon-optimized version of this part, it can be accessed at https://parts.igem.org/Part:BBa_K3273013

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CWChew

Successfully cloned part into S. cerevisiae and tested part by placing nuclease downstream of the galactose inducible promoter Gal1 (Figure 1). Kill switch efficacy was not optimal and had a 82% survival rate.

Attempted to perform part improvement by adding a nuclear localization signal tag to the 5' end of the part sequence to improve the kill switch efficacy in part BBa_K3927015.

T--NUS_Singapore--results_tab3_3.png

Figure 1: Construct schematic of part assembled with a Gal1 promoter upstream to trigger galactose dependent endonuclease activity

T--NUS_Singapore--results_tab3_5.png

Figure 2: CFU assay results from pGNucA-H(BY4741) and pGNLSNucA-H(BY4741), ratio of colonies counted from plates with the nuclease induced to the colonies counted from the plates with nuclease uninduced. Nuclease with the NLS attached reduces the total number of live colonies on the plate.

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UNIQe7fbd8585aa631f5-partinfo-00000002-QINU UNIQe7fbd8585aa631f5-partinfo-00000003-QINU