Tag
MFa1-Secre

Part:BBa_K792002:Experience

Designed by: Manuel Giménez   Group: iGEM12_Buenos_Aires   (2012-09-24)
Revision as of 05:21, 18 October 2021 by CWChew (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K792002

User Reviews

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manugimenez

Successfully used in BBa_K792010, to secrete a Trp-riched peptide. Check BBa_K792010 entry to get more information about the experience.

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CWChew

Successfully expressed GFP using this part to quantify the performance. Characterization of part performance was also done using a GFP reporter. Check NUS Singapore 2021 wiki to view more information: https://2021.igem.org/Team:NUS_Singapore/Results

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Figure 1: Image of the media supernatant, resuspended cells and blank control media under gel visualizer blue light, media and supernatant are clearly fluorescent compared to the control media.

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Figure 2: mFa-GFP construct assembled to test the performance of this secretion tag.

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Figure 3: GFP absorbance of the supernatant and cells of both induced and uninduced pGmFaGFP-H (BY4741), after normalization to blank media. Induced cells secreted approximately 42% of the GFP, while uninduced cells produced and secreted significantly less GFP than induced cells.


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