Coding
Part:BBa_K3952000:Design
Designed by: B. Liam, D. Amanda, R. Jazmine, S. Brianna Group: iGEM21_SUNY_Oneonta (2021-09-30)
Revision as of 04:23, 18 October 2021 by Jazmine124 (Talk | contribs)
APOB-100 (Wild Type)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a positive control of the APOB gene allele that will be used within a Flappase genetic testing system.
A SpaI Restriction Site was found at 259. To adhere to RCF1000 assembly, a silent mutation was applied to the codon located within 257 and 263. The original codon was AGA and was altered to AGG.
Source
The source of the APOB-100 sequence is the genomic sequence from Bos taurus, apolipoprotein B genome. (GeneBank number: NC_037338.1)