Part:BBa_K3952000
APOB-100 (Wild Type)
Located within chromosome 11 of the Bos taurus genome, the APOB gene codes for apolipoprotein B, a protein involved in the transport of cholesterol in the bloodstream. Using the NCBI reference assembly, the APOB gene (Gene ID 494004) was located within the genomic DNA sequence of the Bos taurus. Studies have reported an inheritable allele within the APOB gene that causes cholesterol deficiency in cattle. This part contains the wild-type APOB gene, meaning the allele is absent.
Usage and Biology
The purpose of this part is to serve as a positive control for a Flappase genetic testing system. Therefore the sequence provide is only a fragment of the Bos taurus genome. The sequence was cut in order to only showcase where the allele would be present (exon 5), in addition to the ease of cloning a smaller sequence would provide.
As previously stated there is no allele present in the sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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