Regulatory

Part:BBa_K3875019

Designed by: Yimiao Lin   Group: iGEM21_BUCT   (2021-10-01)
Revision as of 03:54, 18 October 2021 by Ekko (Talk | contribs)


Usage and Biology

It is a bacterial promoter (Registry of Standard Biological Parts BBa_J23119) that modified to end with an SpeI site. J23119 is the "consensus" promoter sequence and the strongest member of the family. The sgRNA will transcribe from the J23119(SpeI) promoter.
Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None