Coding

Part:BBa_K3888004

Designed by: Linhan Lei   Group: iGEM21_SJTang   (2021-10-01)
Revision as of 03:44, 18 October 2021 by RyanLei (Talk | contribs)


HoxK1


HoxK1 coding gene with E. coli signal sequence This basic part is used to achieve heterologous expression of small subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli.[1] HoxK1 encodes the small subunit of hydrogenase mentioned above, and together with HoxG1(BBa_K3888003), to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011). T--SJTang--BBa K3888011-1.png

Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 940
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None