Composite

Part:BBa_K3738013

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)
Revision as of 03:41, 18 October 2021 by Emily Hagens (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


MS2 Bacteriophage Maturation Protein A with N-Terminal Histidine Tag and TEV Site (Composite)

Maturation Protein A is required in order for the capsid of the MS2 phage, and subsequently MS2-derived phage-like particles (PLP), to bind to bacterial pili. It is released from the capsid upon binding, and enters the cell along with the cargo to be delivered by the MS2 capsid. This composite part includes the IPTG-inducible T7 promoter (BBa_J64997), RBS BBa_B0034, maturation protein A coding region (BBa_K3738003), and double terminator BBa_B0015. The maturation protein A coding region includes a histidine tag on the N-terminus for to allow for peptide purification. There is also a TEV cleavage site between the coding region and the histidine tag, in order to allow for histidine tag removal following purification if desired.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 646
    Illegal BamHI site found at 920
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1009


[edit]
Categories
Parameters
None