Coding

Part:BBa_K3570021:Experience

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-10-06)
Revision as of 03:23, 18 October 2021 by Vedantsandhu (Talk | contribs) (→‎Usage)


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Applications of BBa_K3570021

Description

NLS-VP16-EL222 is a blue light-sensitive transcription factor. EL222 contains an LOV domain that forms homodimers in the presence of blue light and induces binding to a C120 consensus DNA motif(TAGCCTTTAGTCCATG). This part has been developed for use in the yeast S.cerevisiae and contains a nuclear localization sequence and VP16 activation factor functional in S.cerevisiae.

Usage

NLS-VP16-EL222 can be expressed in S.cerevisiae as a trans-regulatory factor for a promoter containing a C120 motif in the upstream activation position relative to the core promoter. In blue light, NLS-VP16-EL222 dimerizes and binds to the C120 motif, and a conjugated VP16 domain will promote transcription of downstream elements.

Characterization of expression from an episomal plasmid

User Reviews

UNIQ815e2f6fa642d4d9-partinfo-00000000-QINU UNIQ815e2f6fa642d4d9-partinfo-00000001-QINU