Coding

Part:BBa_K3927006:Design

Designed by: Chew Chin Wei   Group: iGEM21_NUS_Singapore   (2021-10-13)
Revision as of 03:20, 18 October 2021 by Tanianair (Talk | contribs) (→‎Description)


yeLacI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 253
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1044
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 253
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 253
    Illegal NgoMIV site found at 595
  • 1000
    COMPATIBLE WITH RFC[1000]


Description

LacI is a bacterial repressor commonly used in genetic circuits[1]. It has also been shown to be able to repress it’s cognate LacO sequence when expressed in S.cerevisiae[2]. The NUS iGEM 2021 team thus added a yeast optimized LacI to be able to control synthetic promoters in a repressible manner.

Source

NIL

References