Composite

Part:BBa_K3738011:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)
Revision as of 03:16, 18 October 2021 by Registry (Talk | contribs)

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MS2 Bacteriophage Maturation Protein A with N-Terminal Histidine Tag (Composite Part)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 502
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The maturation protein A coding region in this part has been codon-optimized for E.coli, and includes a histidine tag on the N-terminus for to allow for peptide purification.


Source

BBa_J64997, BBa_B0034, BBa_K3738001, BBa_B0015

References