Coding

Part:BBa_K3738008

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)
Revision as of 01:52, 18 October 2021 by Emily Hagens (Talk | contribs)

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MS2 Bacteriophage Capsid Protein Dimer

The MS2 capsid protein comprises most of the capsid of the MS2 phage, as well as MS2 phage-like particles (PLPs). A single MS2 capsid requires 178 copies of the capsid protein, as well as one copy of maturation protein A (BBa_3738000), which is critical in allowing the capsid to bind to the cell and deliver its cargo. This part is a coding region comprised of 2 copies of the MS2 capsid protein, joined by a histidine tag which is flanked by linker sequences (de Martin Garrido et al., 2020).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 138
    Illegal PstI site found at 546
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 138
    Illegal PstI site found at 546
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 138
    Illegal PstI site found at 546
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 138
    Illegal PstI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None