Coding

Part:BBa_K3738003:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)
Revision as of 01:42, 18 October 2021 by Emily Hagens (Talk | contribs) (→‎Design Notes)


MS2 Bacteriophage Maturation Protein A with N-Terminal Histidine Tag and TEV Cleavage Site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 601
    Illegal BamHI site found at 875
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 964


Design Notes

This part is a modified version of BBa_K3738000, to which an N-terminal histidine tag and TEV cleavage site have been added. As maturation protein A has not been well studied for use in phage-like particles, we have designed various versions of the part for testing purposes.

This part has been codon optimized for use in E.coli.

Source

https://www.uniprot.org/uniprot/P03610

References