Composite

Part:BBa_K3809012

Designed by: Francisco Javier Castañeda Villagran   Group: iGEM21_TecCEM   (2021-10-17)
Revision as of 21:38, 17 October 2021 by Registry (Talk | contribs)

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Modified ESR1 protein under strong constitutive promoter and RBS

This sequence is the result of the assembly of two parts: BBa_K3809010 and BBa_K081005. The first one is the coding sequence of the Human Estrogen Receptor Alpha. It was modified in order to express the ligand binding domain and the DNA binding domain. The sequence also includes a 6X Histidine Tag (for its purification), a linker sequence of 4 glycines, 1 serine and 1 cistein (for its immobilization) and a signal peptide sequence (NPS4). The second part is a strong constitutive promoter with an RBS with an efficiency of 0.6. We used this part for the expression of the modified ESR1 protein and its subsequently purification so that we could use it for the detection of Endocrine Disrupting Chemicals or EDCs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 112
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1328


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Categories
Parameters
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