Composite
Part:BBa_K3771076
Designed by: KAO, YI-CHING Group: iGEM21_NCKU_Tainan (2021-10-09)
Revision as of 21:05, 17 October 2021 by Marvinang02 (Talk | contribs)
PlacI-CSAD
Description
PlacI-csad is a composite part consisting of the lacI promoter and the csad sequences. This part was used in in vivo testing of taurine production. The sequence for csad enzyme and lacI promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).
Biology
lacI promoter constitutively facilitates the expression of CSAD enzyme.
圖片描述
Fig. 1 Confirmation of csad fragment by PCR. M: Marker; Lane 1: csad (1368 bp)
Usage
Characterization
Fig. 2 Confirmation of pSAA fragment by digestion. M: Marker; Lane 1: pSAA (2531 bp)
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 476
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 261
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Categories
Parameters
None |