Composite

Part:BBa_K3941005

Designed by: Can Bora Ciner   Group: iGEM21_Saint_Joseph   (2021-10-17)
Revision as of 21:02, 17 October 2021 by Registry (Talk | contribs)

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pET29b+EGII

BBa_K3941005 is a composite part that we used in our expression plasmids (pET29b). We obtain EG2 wild type with this sequence.

This composite part contains 4 parts:

- T7 Promoter (BBa_K3941000)

- LacO (BBa_K1624002)

- EGII (BBa_K3941001)

- T7 Terminator



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 22
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 93
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None