Composite
Part:BBa_K3941005
Designed by: Can Bora Ciner Group: iGEM21_Saint_Joseph (2021-10-17)
pET29b+EGII
BBa_K3941005 is a composite part that we used in our expression plasmids (pET29b). We obtain EG2 wild type with this sequence.
This composite part contains 4 parts:
- T7 Promoter (BBa_K3941000)
- LacO (BBa_K1624002)
- EGII (BBa_K3941001)
- T7 Terminator
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 22
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 93
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 22
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 22
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |