![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3885203
Designed by: Xue Li Group: iGEM21_ZJUT-China (2021-10-10)
P70-ClpXP
Under the influence of the P70 promoter, ClpXP protein can be expressed.The expression of ClpXP in Cell-Free system can improve the degradation rate of protein with ssrA degradation label.
The E. coli complex ClpXP, a protease from the AAA+ family, is an ideal system to achieve targeted protein degradation in Cell-Free reactions by adding, either to the N-terminus or C-terminus, a specifific degron typically 10−12 amino acids long.56 In Cell-Free systems,46 as well as in E. coli, 57 protein degradation by ClpXP is a zeroth order reaction with constant rate.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 448
Illegal EcoRI site found at 1510
Illegal EcoRI site found at 1855 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 448
Illegal EcoRI site found at 1510
Illegal EcoRI site found at 1855 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 448
Illegal EcoRI site found at 1510
Illegal EcoRI site found at 1855
Illegal BglII site found at 1689 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 448
Illegal EcoRI site found at 1510
Illegal EcoRI site found at 1855 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 448
Illegal EcoRI site found at 1510
Illegal EcoRI site found at 1855
Illegal AgeI site found at 1171
Illegal AgeI site found at 1909 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |