Coding

Part:BBa_K3989004

Designed by: Tianyu Xu   Group: iGEM21_UZurich   (2021-09-30)
Revision as of 18:11, 17 October 2021 by LHospital (Talk | contribs)


EsaR D91G mutant

A variant of the Quorum Sensing regulator protein EsaR BBa_K2116001 with the Aspartic acid substituted by Glycine.

Characterisation

According to the literature[1], the amino acid substitution has increased the sensitivity of this protein to 3OC6HSL molecule. The result of our characterisation is shown below and more details can be found in part <a href="https://parts.igem.org/Part:BBa_K3989003"> BBa_K3989003 </a>.

<figure> 

 <img src="21_UZurich_characterisation_plate_reader.jpeg">
 <figcaption>Figure 1. Fluorescence intensity measurement by plate reader(96-well plate). The measurements were done every one hour and this is the curve of the last test.</figcaption>

</figure>

<figure> 

 <img src="21_UZurich_characterisation_facs.jpeg">
 <figcaption>Figure 2. Fluorescence intensity measurement by flow cytometry. The samples are taken from the plate, in which the bacteria has been cultured for 7 hours.</figcaption>

</figure>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

1) Shong, J., Huang, Y. M., Bystroff, C., & Collins, C. H. (2013). Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS chemical biology, 8(4), 789-795.

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