Part:BBa_K3889026
Bacillus subtilis ytvA protein
ytvA protein is a blue light sensor thereby helps in light-induced protein expression.
Usage and Biology
Ytva is continuously produced in the dark state and stays in the dark state until it comes in contact with blue light. It has two domains namely LOV (light oxygen voltage) domain and a C-terminal STAS (sulphate transporter and anti-sigma factor antagonist) domain. LOV domain from these binds to FMN (Flavin Mononucleotide) forming a molecule that has maximum absorbance of 448nm in the dark. Hence, illumination of blue light triggers a photocycle, which includes the formation of a signalling state that converts back to a dark state with a recovery time of 2600s. Ytva-LOV is considered a monomer and is found to exist as a homodimer.
In activated state, YtvA is able to free SigB from RsbW through a cascade of reactions between Regulatory SigB (Rsb) factors as shown:
Through this process, promoters dependent on sigB like BBa_K3889013 starts expressing genes. Overexpression of YtvA can be used to produce system sensitive to blue light.
Parameters
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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