Part:BBa_K3733032

The thiosulfate sensing system under the control of J23103

This part could sense different concentrations of thiosulfate and reflects this difference in fluorescence intensity. Its functionality depends on a two-component system ThsSR, which each constitute a sensor histidine kinase and a response regulator. The ThsS is sensor histidine kinase, which can phosphorylate ThsR in response to thiosulfate. And then the phosphorylated ThsR will binding the site and activate the transcription of P_phsA promoter. In this system, we use J23103 to control the expression of ThsS and ThsR, which in turn controls the differentiation of the system to different thiosulfate concentrations.

The function of BBa_K3733032 is confirmed by iGEM 2021 HZAU-China. See this page:https://parts.igem.org/Part:BBa_K3733032:Experience

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 1151
  Illegal NheI site found at 1867
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 550
  Illegal BamHI site found at 598
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 3065
  Illegal AgeI site found at 3188
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1083
  Illegal SapI.rc site found at 1909