Coding

Part:BBa_K3782006:Design

Designed by: Jana Nf   Group: iGEM21_UNILausanne   (2021-10-17)
Revision as of 16:21, 17 October 2021 by Jana1907 (Talk | contribs) (→‎Design Notes)

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Daucus carota ice-binding protein gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 279
    Illegal BglII site found at 347
    Illegal BglII site found at 824
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 833


Design Notes

The sequence was codon optimized for optimal expression in Escherichia coli.

Source

The source of DcAFP is Daucus carota. Its sequence was retrieved from GenBank. https://www.ncbi.nlm.nih.gov/nuccore/1139877302

References