Protein_Domain

Part:BBa_K3769102

Designed by: Sipeng Chen   Group: iGEM21_FZU-China   (2021-10-17)
Revision as of 15:07, 17 October 2021 by Registry (Talk | contribs)

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Cre-vvd

VVD, a photosensitive protein, is derived from the fungus Neurospora crassa, and homodimerizes under blue light in 400-490nm and separates in the dark. Cre is a commonly used tyrosine recombinase from the P1 bacteriophage that excises DNA flanked by loxP sites.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 141
    Illegal BglII site found at 1021
    Illegal XhoI site found at 855
    Illegal XhoI site found at 1735
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2176
    Illegal AgeI site found at 2659
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None