Part:BBa_K3578000

EXP Promoter

EXP is a strong promoter from Yarrowia lipolytica. It can express genes efficiently. The EXP part will be cloned to a vector according to the iGEM standard design principle to express the glucoamylase or alpha-amylase genes.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 756
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 817
  Illegal SpeI site found at 756
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 756
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 756
  
• 1000
  COMPATIBLE WITH RFC[1000]

Contribution From NNU-China 2021

Group: iGEM Team NNU-China 2021

Author: Yan Xu

Summary: Testing its transcription level

Characterization from iGEM21-NNU-China

        PEXP is an endogenous promoter in Yarrowia lipolytica, which was first registered in 2020. In order to characterize the activity and strength of promoter PEXP, we use the Nluc analysis by constructing the recombinant plasmid pYXLP’-PEXP-Nluc. The constructed recombinant plasmid pYXLP’-PEXP-Nluc was further transformed into Y. lipolytica po1f. Next, we performed the shaking flask to test the Nanoluc luciferase analysis. The experimental result showed a clear fluorescent signal (Fig. 3), demonstrating that the promoter PEXP was able to drive gene transcription in Y. lipolytica and it can be considered as a moderately strong promoter. These results provide references for future iGEM team to select promoters.

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Fig.1 A. The cell growth of strain po1f-PEXP-Nluc P. B. Nanoluc luciferase was used to quantify the strength of promoter PEXP.